dc.contributor.author | Yimer, Solomon Abebe | |
dc.contributor.author | Godana, Alemahehu | |
dc.contributor.author | Kalayou, Shewit | |
dc.contributor.author | Riaz, Tahira | |
dc.contributor.author | Zegeye, Ephrem Debebe | |
dc.contributor.author | Tesfaye Gebeye, Getachew | |
dc.contributor.author | Holm-Hansen, Carol J C | |
dc.contributor.author | Norheim, Gunnstein | |
dc.contributor.author | Abebe, Markose | |
dc.contributor.author | Aseffa, Abraham | |
dc.contributor.author | Tonjum, Tone | |
dc.date.accessioned | 2017-06-16T11:37:44Z | |
dc.date.available | 2017-06-16T11:37:44Z | |
dc.date.created | 2017-05-29T17:04:39Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Frontiers in Microbiology. 2017, 8 795-?. | |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | http://hdl.handle.net/11250/2446265 | |
dc.description.abstract | In order to decipher the nature of the slowly growing Mycobacterium tuberculosis (M.tuberculosis) lineage 7, the differentially abundant proteins in strains ofM. tuberculosis lineage 7 and lineage 4 were defined. Comparative proteomic analysis by mass spectrometry was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages. Label-free peptide quantification of whole cells from M. tuberculosis lineage 7 and 4 yielded the identification of 2825 and 2541 proteins, respectively. A combined total of 2867 protein groups covering 71% of the predicted M. tuberculosis proteome were identified. The abundance of 125 proteins in M. tuberculosis lineage 7 and 4 strains was significantly altered. Notably, the analysis showed that a number of M. tuberculosis proteins involved in growth and virulence were less abundant in lineage 7 strains compared to lineage 4. Five ABC transporter proteins, three phosphate binding proteins essential for inorganic phosphate uptake, and six components of the type 7 secretion system ESX-3 involved in iron acquisition were less abundant in M. tuberculosis lineage 7. This proteogenomic analysis provided an insight into the lineage 7-specific protein profile which may provide clues to understanding the differential properties of lineage 7 strains in terms of slow growth, survival fitness, and pathogenesis. | |
dc.language.iso | eng | |
dc.relation.uri | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00795/full | |
dc.title | Comparative Proteomic Analysis of Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence. | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.source.pagenumber | 795 | |
dc.source.volume | 8 | |
dc.source.journal | Frontiers in Microbiology | |
dc.identifier.cristin | 1472596 | |
dc.relation.project | Norges forskningsråd: 234506 | |
cristin.unitcode | 7502,4,7,0 | |
cristin.unitcode | 7502,4,9,0 | |
cristin.unitname | Infeksjonsimmunologi | |
cristin.unitname | Molekylærbiologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |