dc.contributor.author | Feruglio, Siri | |
dc.contributor.author | Kvale, Dag | |
dc.contributor.author | Dyrhol-Riise, Anne Ma | |
dc.date.accessioned | 2017-06-16T11:53:33Z | |
dc.date.available | 2017-06-16T11:53:33Z | |
dc.date.created | 2017-05-08T14:21:15Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Scandinavian Journal of Immunology. 2017, 85 (2), 138-146. | |
dc.identifier.issn | 0300-9475 | |
dc.identifier.uri | http://hdl.handle.net/11250/2446279 | |
dc.description.abstract | Mycobacterium tuberculosis (Mtb) is particularly challenging for the immune system being an intracellular pathogen, and a variety of T cell subpopulations are activated by the host defence mechanism. In this study, we investigated T cell responses and regulation in active TB patients with drug-sensitive Mtb (N = 18) during 24 weeks of efficient anti-TB therapy. T cell activation, differentiation, regulatory T cell (Treg) subsets, Mtb-induced T cell proliferation and in vitro IL10 and TGF-beta modulation were analysed by flow cytometry at baseline and after 8 and 24 weeks of therapy, while soluble cytokines in culture supernatants were analysed by a 9-plex Luminex assay. Successful treatment resulted in significantly reduced co-expression of HLA-DR/CD38 and PD-1/CD38 on both CD4(+) and CD8(+) T cells, while the fraction of CD4+ CD25 high CD127 low Tregs (P = 0.017) and CD4(+) CD25(high) CD127(low) CD147(+) Tregs (P = 0.029) showed significant transient increase at week 8. In vitro blockade of IL-10/TGF-beta upon Mtb antigen stimulation significantly lowered the fraction of ESAT-6-specific CD4(+) CD25(high) CD127(low) Tregs at baseline (P = 0.047), while T cell proliferation and cytokine production were unaffected. Phenotypical and Mtb-specific T cell signatures may serve as markers of effective therapy, while the IL-10/ TGF-beta pathway could be a target for early inhibition to facilitate Mtb clearance. However, larger clinical studies are needed for verification before concluding. | |
dc.language.iso | eng | |
dc.relation.uri | http://onlinelibrary.wiley.com/doi/10.1111/sji.12511/full | |
dc.title | T Cell Responses and Regulation and the Impact of In Vitro IL-10 and TGF-beta Modulation During Treatment of Active Tuberculosis | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 138-146 | |
dc.source.volume | 85 | |
dc.source.journal | Scandinavian Journal of Immunology | |
dc.source.issue | 2 | |
dc.identifier.doi | 10.1111/sji.12511 | |
dc.identifier.cristin | 1468828 | |
cristin.unitcode | 7502,4,16,0 | |
cristin.unitname | Smitte fra mat, vann og dyr | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |