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dc.contributor.authorPaulsen, Katrine Mørk
dc.contributor.authorStuen, Snorre
dc.contributor.authorDas Neves, Carlos G
dc.contributor.authorSuhel, Faisal
dc.contributor.authorGurung, Deepa
dc.contributor.authorSoleng, Arnulf
dc.contributor.authorStiasny, Karin
dc.contributor.authorVikse, Rose
dc.contributor.authorAndreassen, Åshild Kristine
dc.contributor.authorGranquist, Erik Georg
dc.date.accessioned2021-01-25T08:49:28Z
dc.date.available2021-01-25T08:49:28Z
dc.date.created2019-01-09T16:24:55Z
dc.date.issued2018
dc.identifier.citationZoonoses and Public Health. 2018, 66 (2), 216-222.
dc.identifier.issn1863-1959
dc.identifier.urihttps://hdl.handle.net/11250/2724434
dc.description.abstractTick‐borne encephalitis virus (TBEV) is recognized as the most important zoonotic tick‐transmitted virus in Europe. TBEV is mainly transmitted to humans through bites from TBEV‐infected ticks (Ixodes ricinus and Ixodes persulcatus). However, alimentary infection after consumption of unpasteurized milk and cheese from domestic ruminants has been reported. There is little information about TBEV in ruminants in Norway. The objectives of this study were to analyse unpasteurized cow milk for TBEV RNA and to study the presence of IgG antibodies to TBEV in the same animals. A total of 112 milk and blood samples were collected from cows from five different farms spread from southern to northern Norway. The milk samples were analysed by an in‐house reverse transcription (RT) real‐time polymerase chain reaction and confirmed by pyrosequencing. Serum samples were screened by a commercial enzyme‐linked immunosorbent assay and verified by a TBEV‐specific serum neutralization test. We found TBEV RNA in unpasteurized milk collected from farms in the municipalities of Mandal, Skedsmo and Brønnøy in 5.4% of the tested animals. Specific antibodies to TBEV were only detected in Arendal, where 88.2% of the tested animals were positive. Further studies on milk containing TBEV RNA should be performed to conclude if TBEV found in unpasteurized milk in Norway is infectious, which could be of great importance in a One Health perspective.
dc.language.isoeng
dc.titleTick-borne encephalitis virus in cows and unpasteurized cow milk from Norway
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.pagenumber216-222
dc.source.volume66
dc.source.journalZoonoses and Public Health
dc.source.issue2
dc.identifier.doi10.1111/zph.12554
dc.identifier.cristin1653557
dc.relation.projectHelse- og omsorgsdepartementet: B1214
dc.relation.projectEU/167226
dc.relation.projectEU/20200422
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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