dc.contributor.author | Ueland, Grethe Åstrøm | |
dc.contributor.author | Kellmann, Ralf | |
dc.contributor.author | Davidsen, Melissa Jørstad | |
dc.contributor.author | Viste, Kristin | |
dc.contributor.author | Husebye, Eystein Sverre | |
dc.contributor.author | Almås, Bjørg | |
dc.contributor.author | Storr, Helen L. | |
dc.contributor.author | Sagen, Jørn Vegard | |
dc.contributor.author | Mellgren, Gunnar | |
dc.contributor.author | Juliusson, Pétur Benedikt | |
dc.contributor.author | Methlie, Paal | |
dc.date.accessioned | 2022-07-26T13:24:55Z | |
dc.date.available | 2022-07-26T13:24:55Z | |
dc.date.created | 2021-05-20T15:32:13Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Journal of the Endocrine Society (JES). 2021, 5 (5), 1-8. | |
dc.identifier.issn | 2472-1972 | |
dc.identifier.uri | https://hdl.handle.net/11250/3008653 | |
dc.description.abstract | Abstract Background:Diagnosing Cushing syndrome (CS) can be challenging. The 24-hour urine free cortisol (UFC) measurement is considered gold standard. This is a laborious test, de-pendent on correct urine collection. Late-night salivary cortisol is easier and is used as a screening test for CS in adults, but has not been validated for use in children. Objective:To define liquid chromatography tandem mass spectrometry (LC-MS/MS)-based cutoff values for bedtime and morning salivary cortisol and cortisone in children, and validate the results in children with and without CS. Methods:Bedtime and morning salivary samples were collected from 320 healthy chil-dren aged 4 to 16 years. Fifty-four patients from the children’s outpatient obesity clinic and 3 children with pituitary CS were used for validation. Steroid hormones were as-sayed by LC-MS/MS. Cutoff levels for bedtime salivary cortisol and cortisone were de-fined by the 97.5% percentile in healthy subjects. Results:Bedtime cutoff levels for cortisol and cortisone were 2.4 and 12.0 nmol/L, re-spectively. Applying these cutoff levels on the verification cohort, 1 child from the obesity clinic had bedtime salivary cortisol exceeding the defined cutoff level, but normal sal-ivary cortisone. All 3 children with pituitary CS had salivary cortisol and cortisone far above the defined bedtime cutoff levels. Healthy subjects showed a significant decrease in salivary cortisol from early morning to bedtime. | |
dc.language.iso | eng | |
dc.title | Bedtime Salivary Cortisol as a Screening Test for Cushing Syndrome in Children | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 1-8 | |
dc.source.volume | 5 | |
dc.source.journal | Journal of the Endocrine Society (JES) | |
dc.source.issue | 5 | |
dc.identifier.doi | 10.1210/jendso/bvab033 | |
dc.identifier.cristin | 1911115 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |