dc.contributor.author | Pradhan, Brajabandhu | |
dc.contributor.author | Liedtke, Janine | |
dc.contributor.author | Sleutel, Mike | |
dc.contributor.author | Lindbäck, Toril | |
dc.contributor.author | Zegeye, Ephrem Debebe | |
dc.contributor.author | O_Sullivan, Kristin | |
dc.contributor.author | Llarena, Ann-Katrin | |
dc.contributor.author | Brynildsrud, Ola Brønstad | |
dc.contributor.author | Aspholm, Marina Elisabeth | |
dc.contributor.author | Remaut, Han | |
dc.date.accessioned | 2022-08-01T08:43:56Z | |
dc.date.available | 2022-08-01T08:43:56Z | |
dc.date.created | 2021-06-28T16:11:51Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | EMBO Journal. 2021, . | |
dc.identifier.issn | 0261-4189 | |
dc.identifier.uri | https://hdl.handle.net/11250/3009521 | |
dc.description.abstract | Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by β-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores. | |
dc.language.iso | eng | |
dc.title | Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 16 | |
dc.source.journal | EMBO Journal | |
dc.identifier.doi | 10.15252/embj.2020106887 | |
dc.identifier.cristin | 1919060 | |
dc.relation.project | Norges forskningsråd: 249069 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |