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dc.contributor.authorSharma, Supriya
dc.contributor.authorAcharya, Jyoti
dc.contributor.authorCaugant, Dominique A
dc.contributor.authorBanjara, Megha Raj
dc.contributor.authorGhimire, Prakash
dc.contributor.authorSingh, Anjana
dc.date.accessioned2022-08-01T09:05:22Z
dc.date.available2022-08-01T09:05:22Z
dc.date.created2021-05-25T11:17:05Z
dc.date.issued2021
dc.identifier.citationInfectious Disease Reports. 2021, 13 173-180.
dc.identifier.issn2036-7430
dc.identifier.urihttps://hdl.handle.net/11250/3009554
dc.description.abstractThe rapid identification of bacteria causing meningitis is crucial as delays in the treatment increase mortality rate. Though considered as the gold standard for the laboratory diagnosis of bacterial meningitis, culture might give false negative results in a case of patients under antibiotics prior to lumbar puncture. This study aimed to detect Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae by a multiplex polymerase chain reaction (PCR) in culture-negative cerebrospinal fluid samples collected from clinically suspected meningitis cases attending different hospitals in Kathmandu, Nepal from January 2017 to December 2019. S. pneumoniae, N. meningitidis and H. influenzae were detected in 8.59% (33/384) of the specimens by PCR and 7.55% (29/384) of the specimens by culture. Correlation between culture and PCR of the same sample was good (Spearman’s rho correlation coefficient = 0.932). However, the difference in positivity between culture and PCR was statistically not significant (p value > 0.05). In four specimens, culture could not detect any of the targeted bacteria whereas PCR could detect presence of H. influenzae. PCR increases the diagnostic yield for bacterial meningitis. PCR may be considered as an adjunctive test for establishing the cause of infection in culture negative clinically suspected meningitis cases.
dc.language.isoeng
dc.titleDetection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae in Culture Negative Cerebrospinal Fluid Samples from Meningitis Patients Using a Multiplex Polymerase Chain Reaction in Nepal
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.pagenumber173-180
dc.source.volume13
dc.source.journalInfectious Disease Reports
dc.identifier.doi10.3390/idr13010019
dc.identifier.cristin1911640
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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