• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Folkehelseinstituttet
  • Publikasjoner fra CRIStin FHI
  • View Item
  •   Home
  • Folkehelseinstituttet
  • Publikasjoner fra CRIStin FHI
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput

Rundén-Pran, Elise; Mariussen, Espen; El Yamani, Naouale; Elje, Elisabeth; Longhin, Eleonora Marta
Peer reviewed, Journal article
Published version
Thumbnail
View/Open
Runden-Pran+et+al_Front+Toxicol_2022.pdf (1.089Mb)
URI
https://hdl.handle.net/11250/3024877
Date
2022
Metadata
Show full item record
Collections
  • Artikler [6810]
  • Publikasjoner fra CRIStin FHI [9384]
Original version
Frontiers in Toxicology. 2022, 4 .   10.3389/ftox.2022.983316
Abstract
To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for in vitro toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with the read-out or the reagent of the assay needs to be addressed to avoid biased results. Thus, validated label-free methods are urgently needed for hazard identification of NMs to avoid unintended adverse effects on human health. The colony forming efficiency (CFE) assay is a label- and interference-free method for quantification of cytotoxicity by cell survival and colony forming efficiency by CFE formation. The CFE has shown to be compatible with toxicity testing of NMs. Here we present an optimized protocol for a higher-throughput set up.
 
The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput
 
Journal
Frontiers in Toxicology

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit